Home Antibody All anti-CENPH antibodies
Anti-CENPH TRUEMAB Antibody clone 2A11
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for CENPH (NM_022909)
|Full length human recombinant protein of human CENPH(NP_075060) produced in HEK293T cell.|
|WB, IF, FC
|| WB 1:2000, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens centromere protein H (CENPH)|
|NNF1; PMF1;PMF1; NNF1, MIND kinetochore complex component, homolog; kinetochore protein CENP-H; centromere protein H|
Entrez Gene 64946 Human
|Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008]. |
|Druggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CENPH (RC204531, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CENPH.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CENPH monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-CENPH mouse monoclonal antibody (TA503912) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CENPH(RC204531).
HEK293T cells transfected with either RC204531 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CENPH antibody(TA503912), and then analyzed by flow cytometry.