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Home Antibody All anti-CDK9 antibodies

Anti-CDK9 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319415
  • Rabbit polyclonal anti-Cdk9 antibody
100ul 325 3-7 Days
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WB(1)
IF(1)

OriGene Data

ImmunogenMultiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE).
Clone Name IsotypeIgG
Species Reactivityhuman, rat and mouse ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsELISA: 1:10,000 - 1:50,000, WB: 1:500 - 1:3,000, IHC: 1:200 - 1:1,000, IP: 1:100
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2.   This kinase also was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. Tat stimulates human HIV-1 viral transcription elongation.  This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation.  Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb.   The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev.  CDK9 (PITALRE) is a ubiquitously expressed nuclear protein.

Reference Data

Target NameHomo sapiens cyclin-dependent kinase 9 (CDK9)
Alternative NameC-2k; CDC2L4; CTK1; PITALRE; TAK
Database LinkNP_001252
Entrez Gene 1025 Human
Entrez Gene 107951 Mouse
Entrez Gene 362110 Rat
Function
Related PathwayProtein KinaseTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
anti cdk9 antibody (TA319415 1:1500 ) was used for Western blot analysis of 1) PC3 and 2) DU145 prostate cancer cells (50ug per lane). Bands at the expected MW of 55 and 42 Kda were detected. Personal communication Flavio Rizzolio, Temple University
IF Image
Immunocytochemical staining of mouse tissue using anti-cdk9 (Pitalre) antiserum.  The staining shows the location of mcdk9/Pitalre protein in developing mouse tissue. Arrows indicate areas of high expression.  Panel A: Peroxidase-DAB immunostaining of mcdk9/Pitalre protein in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle.  Similar staining is shown in Panel B in the dorsal root ganglia.   Panel C:  Fluorescein immunofluorescence of mcdk9/Pitalre in skeletal muscle.   Similar staining is shown in Panel D in cardiac muscle.   Other detection systems should yield similar results.  Sections from each specimen were cut at 5-7 µm, mounted on glass and dried overnight at 37°C. All sections then were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS). PBS was used for all subsequent washes and for antiserum dilution. Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum. Slides were incubated at 20° C for 1 h  with  rabbit anti-cdk9 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min. All the slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20° C.  Diaminobenzidine was used as the final chromogen and hematoxylin was used as the nuclear counterstain. Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.

 

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