Home All anti-CDC42 antibodies
Anti-CDC42 Phospho Antibody EP221Y
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for CDC42 (NM_001791)
|A phospho-specific peptide corresponding to residues surrounding Serine 71 of human Rac1/Cdc42 was used as an immunogen. The antibody only detects Rac1/Cdc42 phosphorylated on Serine 71.|
||WB: 1:500~1000; ICC: 1: 100~250
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens cell division cycle 42 (CDC42), transcript variant 1|
|Rac1 and cell division cycle 42 (cdc42) are guanosine triphosphatases (GTPases). Rac1 is involved in signal transduction pathways that induce the formation of lamellipodia, stimulate cell proliferation and activate the JNK/SAPK protein kinase cascade. Rac1 regulates unique aspects of hematopoietic development and function, and is critical for the hypertrophic response in the heart. Therapies that target myocardial Rac1 are reported to be beneficial in the treatment of cardiac hypertrophy. Cdc42 has been shown in endothelial cell monolayers to prevent disassembly of interendothelial junctions and the increase in endothelial permeability. Cdc42 activity of microvessel endothelial cells is a critical determinant of junctional barrier restrictiveness and may represent a means of therapeutically modulating increased lung vascular permeability and edema formation. The phosphorylation of Rac1 inhibits its GTP-binding activity without any significant change in GTPase activity. Akt phosphorylates serine 71 of Rac1, modulating the Rac1 signal transduction pathway (1-5). |
EGFR1 Signaling Pathway
MAPK signaling pathway
Signaling by GPCR
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot analysis on A431 cell lysates using anti-Phospho-Rac1/Cdc42 (pS71), 1:1000 dilution. Cells were either (A) treated or (B) untreated with Lambda Phosphatase.
Immunofluorescent analysis of A431-2 using anti-Phospho-Rac1/Cdc42 (pS71).