Anti-CD5 Antibody, clone Clone UMAB9

Also for CD5 (NM_014207)

Gene Name	Homo sapiens CD5 molecule (CD5)
Synonyms	LEU1; T1
Immunogen	Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	Clone UMAB9	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Background	CD5 is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. B-1 cells have limited diversity of theirB-cell receptor due to their lack of the enzyme terminal deoxynucleotidyl transferase (TdT) and are potentially self-reactive. CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins. CD5 is a good immunohistochemical marker for T-cells. About 76% of T-cell neoplasms are reported to express CD5, and it is also found in chronic lymphocytic leukemia, hairy cell leukaemia, and mantle cell lymphoma cells. It is commonly lost in cutaneous T-cell lymphoma, and its absence can be used as an indicator of malignancy in this condition. The absence of CD5 in T cell acute lymphoblastic leukaemia, while relatively rare, is associated with a poor prognosis.

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WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD5 monoclonal antibody (Clone UMAB9) at 1:500.

IHC Image

Immunohistochemical staining of paraffin-embedded lymph node tissue using anti-CD5 mouse monoclonal antibody. (Clone UMAB9, Dilution 1:100)

IHC Image

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Clone UMAB9, Dilution 1:100)

IHC Image

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD5 mouse monoclonal antibody. (Clone UMAB9, Dilution 1:100)

IF Image

Anti-CD5 mouse monoclonal antibody (UM500009) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD5(RC206494).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(UM500009),(Red), compared to a nonspecific negative control antibody,(Blue).

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
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PE
FITC
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DyLight® 633

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Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

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