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Anti-CCNE1 PHOSPHO Antibody
Also for CCNE1 (NM_001238)
|The antiserum was produced against synthesized phosphopeptide derived from human Cyclin E1 around the phosphorylation site of threonine 395 (L-L-TP-P-P).|
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|Cyclin E1 (phospho-Thr395) antibody detects endogenous levels of Cyclin E1 only when phosphorylated at threonine 395
|Homo sapiens cyclin E1 (CCNE1)|
TGF Beta Signaling Pathway
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Western blot analysis of extracts from HeLa cells, treated with Paclitasel (1uM, 60mins), using Cyclin E1 (phospho-Thr395) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Cyclin E1 (phospho-Thr395) antibodyThe picture on the right is treated with the synthesized peptide.