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Home Antibody All anti-CCND1 antibodies

Anti-CCND1 Antibody EPR2241

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA301123 Rabbit Monoclonal Antibody against CCND1 (Clone EPR2241IHC) 100ul $325 In Stock
LC403284 CCND1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)
IHC(1)
Assay(1)
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Also for CCND1 (NM_053056)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Cyclin D1 was used as an immunogen.
Clone NameEPR2241 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, Assay, IHC Suggested DilutionsWB: 1:10000; IP: 1:40; ICC/IF: 1:100; IHC-P: 1:100
Predicted MW Explanation 34 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens cyclin D1 (CCND1)
Alternative NameBCL1; D11S287E; PRAD1; U21B31
Database LinkNP_444284
Entrez Gene 595 Human
Entrez Gene 12443 Mouse
Entrez Gene 58919 Rat
FunctionCyclin D1 belongs to the Cyclin D family. Cyclin D1 is required for the cell cycle G1/S transition (1). Cyclin D1 forms a serine/threonine kinase holoenzyme complex with and functions as a regulatory subunit of CDK4 or CDK6. Cyclin D1 also phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1/S phase (2-3). Amplification or overexpression of cyclin D1 plays a pivotal role in the development of various human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer (4). Over-expression of cyclin D1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis (5). Cyclin D1 also associates with, and regulates the activity of transcription factors, coactivators and corepressors that govern histone acetylation and chromatin remodeling proteins (6-7)
Related PathwayStem cell relevant signaling - JAK/STAT signaling pathwayStem cell relevant signaling - Wnt Signaling pathwayStem cell - PluripotencyStem cell relevant signaling - DSL/Notch pathwayDruggable Genome Cell cyclep53 signaling pathwayWnt signaling pathwayFocal adhesionJak-STAT signaling pathwayPathways in cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-Cyclin D1 antibody [EPR2241]; .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 34 kDa.Exposure time : 3 minutes
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Western blot - Anti-Cyclin D1 antibody [EPR2241]; Anti-Cyclin D1 antibody [EPR2241] at 1/10000 dilution + MCF7 cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 34 kDa.
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Other-Anti-Cyclin D1 antibody [EPR2241](TA301123); Equilibrium disassociation constant (KD)..
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [EPR2241]; Immunohistochemical analysis of paraffin-embedded Human mantle cell lymphoma tissue labelling Cyclin D1 with TA301123 at 1/100 dilution.
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