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Anti-CCNB1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for CCNB1 (NM_031966)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein.|
|Human , Dog , Rat , Monkey , Mouse
|WB, ELISA, IHC
|| WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens cyclin B1 (CCNB1)|
Entrez Gene 891 Human
Entrez Gene 268697 Mouse
Entrez Gene 25203 Rat
Entrez Gene 699504 Monkey
Entrez Gene 608420 Dog
|In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is Ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.|
Hedgehog signaling pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNB1 (RC200812, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNB1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CCNB1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CCNB1 rabbit polyclonal antibody. (TA590439)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CCNB1 rabbit polyclonal antibody. (TA590439)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CCNB1 rabbit polyclonal antibody. (TA590439)