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Home Antibody All anti-CCNB1 antibodies
UltraMAB

Anti-CCNB1 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590439 Rabbit Polyclonal Cyclin B1 Antibody 100ug $325 In Stock
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WB(2)
IHC(3)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the N Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration0.81168 mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens cyclin B1 (CCNB1)
Alternative NameCCNB
Database LinkNP_114172
Entrez Gene 891 Human
Entrez Gene 268697 Mouse
Entrez Gene 25203 Rat
Entrez Gene 699504 Monkey
Entrez Gene 608420 Dog
FunctionIn mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is Ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Related PathwayStem cell - PluripotencyDruggable Genome Cell cycleOocyte meiosisp53 signaling pathwayProgesterone-mediated oocyte maturation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNB1 (RC200812, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNB1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CCNB1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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ELISA: Cyclin B1 Antibody
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CCNB1 rabbit polyclonal antibody. (TA590439)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CCNB1 rabbit polyclonal antibody. (TA590439)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CCNB1 rabbit polyclonal antibody. (TA590439)
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