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Anti-CBL PHOSPHO Antibody EP2225Y
|A phospho specific peptide corresponding to residues surrounding tyrosine 700 were used as an immunogen. This antibody detects Cbl phosphorylated on tyrosine 700.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 ; IP: 1:30;
|Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.|
|Homo sapiens Cbl proto-oncogene, E3 ubiquitin protein ligase (CBL)|
|C-CBL; CBL2; FRA11B; NSLL; RNF55|
|c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4). It has been demonstrated that tyrosines 700, 731, and 774 are the major tyrosine phosphorylation sites of c-Cbl in T cells in response to pervanadate treatment, as well as in response to TcR/CD3 ligation (5).|
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
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All lanes : Anti-CBL (phospho Y700) antibody [EP2225Y] (TA310244) at 1/10000 dilution. Lane 1 : Jurkat cell lysate, untreated; Lane 2 : Jurkat cell lysate, treated with pervanadate. Lysates/proteins at 10 µg per lane. Secondary: HRP labelled goat anti-rabbit at 1/2000 dilution.