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Anti-CASP3 Antibody E61
Also for CASP3 (NM_032991)
|A synthetic peptide correscponding to N-terminal residues of Human Pro-Caspase-3 was used as immunogen. The antibody only recognizes the pro-form of Caspase-3. It does not react with the cleaved forms (active enzyme) of Caspase-3. This antibody is predict|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||FC: 1:100; IP: 1:10; WB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta|
|CPP32; CPP32B; SCA-1|
|Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).|
MAPK signaling pathway
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Western blot - pro Caspase 3 antibody [E61]; Anti-pro Caspase 3 antibody [E61] at 1/1000 dilution + Hela cell lysate.Observed band size : 35 kDa .
Immunohistochemistry (Paraffin-embedded sections) - pro Caspase 3 antibody [E61]; Immunohistochemical analysis of human paraffin-embedded cervical carcinoma tissue using ab32150 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - pro Caspase 3 antibody [E61]; Immunofluorescent staining of HeLa cells using ab32150 at 1/100 dilution.
Flow Cytometry-Anti-pro Caspase 3 antibody [E61](ab32150); Overlay histogram showing Jurkat cells stained with ab32150 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (1µg/1x10^6 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.