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Anti-CAMK2A PHOSPHO Antibody EPR1830
Also for CAMK2A (NM_015981)
|A phospho specific peptide corresponding to residues surrounding Threonine 286 of human CaMKII was used as an immunogen. This antibody only detects CaMKII phosphorylated at Threonine 286.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens calcium/calmodulin-dependent protein kinase II alpha (CAMK2A), transcript variant 1|
Entrez Gene 815 Human
Entrez Gene 12322 Mouse
Entrez Gene 25400 Rat
|Calcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. It is a ubiquitous serine/threonine protein kinase which is abundant in brain as a major PSD constituent. This enzyme is composed of distinct but related subunits (alpha, beta, gamma, and delta) (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex is involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2). The most important autophosphorylation site is Threonine 286, which resides in the vicinity of the autoinhibitory domain (3).|
|Protein KinaseDruggable Genome ErbB signaling pathwayCalcium signaling pathwayOocyte meiosisWnt signaling pathwayLong-term potentiationNeurotrophin signaling pathwayMore Pathways >> |
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Western blot - Anti-CaMKII alpha (phospho T286) antibody [EPR1830]; All lanes : Anti-CaMKII alpha (phospho T286) antibody [EPR1830] at 1/1000 dilution.Lane 1 : Rat brain lysate untreated.Lane 2 : Rat brain lysate treated with Lambda Phosphatase.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 54 kDa.
Other-Anti-CaMKII alpha (phospho T286) antibody [EPR1830](TA310229); Equilibrium disassociation constant (KD)..