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Anti-CAMK2A Antibody EP1829Y
Also for CAMK2A (NM_015981)
|A synthetic peptide corresponding to residues on human CaMKII was used as an immunogen|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:20000; IP: 1:50; ICC: 1:250 - 1:500; FC: 1:80; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens calcium/calmodulin-dependent protein kinase II alpha (CAMK2A), transcript variant 1|
|The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]. |
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - CaMKII antibody [EP1829Y]; Anti-CaMKII antibody [EP1829Y] at 1/20000 dilution + Mouse brain lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 54 kDa.Observed band size : 45,70 kDa .
Immunohistochemistry (Paraffin-embedded sections) - CaMKII antibody [EP1829Y]; Immunohistochemical staining of paraffin-embedded human brain carcinoma using ab52476 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - CaMKII antibody [EP1829Y]; ICC/IF image of ab52476 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43?M.