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Home Antibody All anti-CA9 antibodies

Anti-CA9 TRUEMAB Antibody clone 1G7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500623
  • anti-CA9 (Carbonic Anhydrase IX) mouse monoclonal antibody, clone 1G7
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400485) , 20ug Explanation
100ul 379 In Stock
CF500623
  • Carrier-free (BSA/glycerol-free) CA9 mouse monoclonal antibody, clone 1G7
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IHC(1)
FC(1)
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Also for CA9 (NM_001216)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human CA9 expression vector
Clone Nameclone 1G7 IsotypeIgG2b
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:50, Flow 1:100
Predicted MW Explanation 49.7 KDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens carbonic anhydrase IX (CA9)
Alternative NameCAIX; MN
Database LinkNP_001207
Entrez Gene 768 Human
FunctionCarbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-basebalance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
Related PathwayTransmembraneDruggable Genome Nitrogen metabolism

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CA9 (RC204839, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CA9.
IHC Image
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-CA9mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500623, Dilution 1:50)
FC Image
HEK293T cells transfected with either pCMV6-ENTRY CA9(RC204839)(Red) or empty vector control plasmid(Blue) were immunostained with anti-CA9 mouse monoclonal(TA500623, Dilution 1:1,000), and then analyzed by flow cytometry.

 

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