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Home Antibody All anti-CA12 antibodies

Anti-CA12 TRUEMAB Antibody Clone OTI2E9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA505570 CA12 mouse monoclonal antibody, clone OTI2E9 (formerly 2E9) 100ul $325 In Stock
LC400487 CA12 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF505570 Carrier-free (BSA/glycerol-free) CA12 mouse monoclonal antibody, clone OTI2E9 (formerly 2E9) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IF(2)

OriGene Data

ImmunogenFull length human recombinant protein of human CA12(NP_001209) produced in HEK293T cell.
Clone NameClone OTI2E9 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IF Suggested Dilutions WB 1:2000, IF 1:100,
Predicted MW Explanation 36.8 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens carbonic anhydrase XII (CA12), transcript variant 1
Alternative NameCAXII; HsT18816
Database LinkNP_001209
Entrez Gene 771 Human
FunctionCarbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq].
Related PathwayTransmembraneDruggable Genome Nitrogen metabolism

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CA12 (RC204810, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CA12.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CA12 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Anti-CA12 mouse monoclonal antibody (TA505570) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CA12(RC204810).
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Immunofluorescent staining of HeLa cells using anti-CA12 mouse monoclonal antibody (TA505570).
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