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Anti-C11orf30 Antibody Y474


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against EMSY, Isoform1(Y474)
100ul $325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-term of human EMSY isoform 1 was used as immunogen.
Clone NameY474 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:1000; ICC/IF: 1:250 - 1:500; FC: 1:150; IP: 1:50
Predicted MW Explanation 141 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Is unsuitable for IHC.

Reference Data

Target NameHomo sapiens chromosome 11 open reading frame 30 (C11orf30), transcript variant 4
Alternative NameEMSY; GL002
Database LinkNP_064578
Entrez Gene 56946 Human
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - EMSY antibody [Y474]; Anti-EMSY antibody [Y474] at 1/1000 dilution + MCF7 cell lysate.Predicted band size : 141 kDa.Observed band size : 150 kDa .
Immunocytochemistry/ Immunofluorescence - EMSY antibody [Y474]; Immunofluorescent analysis of EMSY expression in MCF7 cells using 1/250 TA303798.
Flow Cytometry-Anti-EMSY antibody [Y474](TA303798); Overlay histogram showing MCF7 cells stained with TA303798 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


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