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Anti-C11orf30 Antibody Y474
Also for C11orf30 (NM_020193)
|A synthetic peptide corresponding to residues near the C-term of human EMSY isoform 1 was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IF, FC
||WB: 1:1000; ICC/IF: 1:250 - 1:500; FC: 1:150; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for IHC.
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Western blot - EMSY antibody [Y474]; Anti-EMSY antibody [Y474] at 1/1000 dilution + MCF7 cell lysate.Predicted band size : 141 kDa.Observed band size : 150 kDa .
Immunocytochemistry/ Immunofluorescence - EMSY antibody [Y474]; Immunofluorescent analysis of EMSY expression in MCF7 cells using 1/250 TA303798.
Flow Cytometry-Anti-EMSY antibody [Y474](TA303798); Overlay histogram showing MCF7 cells stained with TA303798 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.