Anti-BRAF Antibody, clone 1E2

Also for BRAF (NM_004333)

Gene Name	Homo sapiens v-raf murine sarcoma viral oncogene homolog B1 (BRAF)
Synonyms:	B-RAF1; BRAF1; NS7; RAFB1
Immunogen	Full length human recombinant protein of human BRAF (NP_004324) produced in HEK293T cell.
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 1E2	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
Background	This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
Related Pathway	Focal Adhesion MAPK signaling pathway Senescence and Autophagy

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (TA500845, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-BRAFmouse monoclonal antibody. (TA500845, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded liver tissue using anti-BRAFmouse monoclonal antibody. (TA500845, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BRAFmouse monoclonal antibody. (TA500845, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-BRAFmouse monoclonal antibody. (TA500845, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-BRAFmouse monoclonal antibody. (TA500845, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-BRAFmouse monoclonal antibody. (TA500845, Dilution 1:50)

IF Image

Anti-BRAF mouse monoclonal antibody (TA500845) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF(RC211013).

IF Image

Immunofluorescent staining of HepG2 cells using anti-BRAF mouse monoclonal antibody (TA500845).

FC Image

HEK293T cells transfected with either pCMV6-ENTRY BRAF(RC211013)(Red) or empty vector control plasmid(Blue) were immunostained with anti-BRAF mouse monoclonal(TA500845), and then analyzed by flow cytometry.

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Anti-BRAF TrueMAB Antibody clone 1E2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens