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OriGene Antibodies in recent publications
Novel HIF2A mutations disrupt oxygen sensing, leading to polycythemia, paragangliomas, and somatostatinomas Blood, Mar 2013; 121: 2563 - 2566. [anti-HA]

Regulation of the PI3-K/Akt Survival Pathway in the Rat Endometrium Biol Reprod, Mar 2013; 88: 79. [Akt3]

RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP Nucleic Acids Res., Mar 2013; 10.1093/nar/gkt159. [LA]

Ruxolitinib as potential targeted therapy for patients with JAK2 rearrangements Haematologica, Mar 2013; 98: 404 - 408. [JAK2]

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Home All anti-BRAF antibodies

Anti-BRAF TrueMAB Antibody clone 3B12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Related Products Conjugation/Bulk FAQs
SKU Description Amount Price Availability*  
TA500446
  • Purified anti-BRAF mouse monoclonal antibody,clone 3B12
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401382) , 20ug
100ul $325 In Stock Add to Shopping Cart
CF500446
  • Carrier-free (BSA/glycerol-free) BRAF mouse monoclonal antibody,clone 3B12
100ug $450 3-4 weeks Add to Shopping Cart
WB(2)
IHC(4)
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Also for BRAF (NM_004333)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens v-raf murine sarcoma viral oncogene homolog B1 (BRAF)
Synonyms:B-RAF1; BRAF1; NS7; RAFB1
ImmunogenProtein expressed in 293T cell transfected with human BRAF expression vector
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Nameclone 3B12 IsotypeIgG1
Species ReactivityHuman, Dog, Monkey Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC Suggested DilutionsWB 1:2000 IHC 1:50
BackgroundThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases.This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation,and secretion. Mutations in this ge
Related Pathway
Focal Adhesion
MAPK signaling pathway
Senescence and Autophagy

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-BRAFmonoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BRAFmouse monoclonal antibody. (TA500446, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-BRAFmouse monoclonal antibody. (TA500446, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-BRAFmouse monoclonal antibody. (TA500446, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-BRAFmouse monoclonal antibody. (TA500446, Dilution 1:50)

 

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