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Home Antibody All anti-BIN1 antibodies

Anti-BIN1 Antibody 99F

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SKU Description Amount Price Availability*  
TA319580
  • Mouse monoclonal anti-BIN1 antibody
100ug 325 In Stock
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WB(1)
IP(1)
IF(1)
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Also for BIN1 (NM_004305)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenAnti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with BIN1 polypeptide followed by hybridoma development.
Clone Name99F Isotype
Species Reactivitymouse, human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, IP Suggested DilutionsELISA: 1:5000-1:50000, WB: 1:500-1:1500, IHC: 1:100-1:500, IP: 10-100 uL
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells and play a role in cancer suppression. Studies in muscle cells suggest that Bin1 expression, structure, and localization are tightly regulated during muscle differentiation and suggested that Bin1 plays a functional role in the differentiation process. Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive; also known as autosomal recessive myotubular myopathy.

Reference Data

Target NameHomo sapiens bridging integrator 1 (BIN1), transcript variant 8
Alternative NameAMPH2; AMPHL; SH3P9
Database LinkNP_004296
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western Blot of Mouse Anti-BIN1 antibody. Lane 1: C2C12 during growth. Lane 2: C2C12 during differentiation. Load: 35 µg per lane. Primary antibody: BIN1 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 64.7 kDa, ~55 kDa for BIN-1. Other band(s): non-specifics.
IF Image
Immunofluorescence Microscopy of Mouse Anti-BIN1 Antibody. Cells: C2C12 cells during growth or differentiation. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN 1 as green fluorescent signal.
IP Image
Immunoprecipitation of Mouse anti-BIN1 antibody. Immunoprecipitates were separated by SDS-PAGE and visualized by fluorography. The differentiation-associated (Exon 10+) form of Bin1 is recognized by using Bin1 (Exon 10 specific, 99F) monoclonal antibody. Lane 1: BIN 1 Lane 2: none. Load: 35 µg per lane. Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 64.7 kDa, ~65 kDa for BIN-1. Other band(s): none.

 

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