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Anti-BGN Antibody EPR2959
|A synthetic peptide corresponding to residues in C-terminus of human Biglycan was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:100 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for ICC or IHC-P.
|Homo sapiens biglycan (BGN)|
|DSPG1; PG-S1; PGI; SLRR1A|
|Biglycan is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. Biglycan and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein plays a role in assembly of collagen fibrils and muscle regeneration. It interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI, and it is critical for the assembly of the dystrophin-associated protein complex (1).|
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Western blot - Biglycan antibody [EPR2959]; All lanes : Anti-Biglycan antibody [EPR2959] at 1/1000 dilution.Lane 1 : Rat cartilage lysate.Lane 2 : L6 lysate.Lane 3 : C2C12 lysate.Lane 4 : Human skeletal muscle lysate.Lane 5 : Mouse muscle lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 42 kDa.
Flow Cytometry - Biglycan antibody [EPR2959]; Flow cytometric analysis of permeabilized L6 cells using TA307856 at 1/100 dilution (red) or a rabbit IgG (negative) (green).