Anti-BEST3 Antibody, clone 2H3

Also for BEST3 (NM_152439)

OriGene Data

Immunogen	Full length human recombinant protein of human BEST3 (NP_689652) produced in HEK293T cell.
Clone Name	clone 2H3	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:2000, IF 1:100, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens bestrophin 3 (BEST3), transcript variant 2
Alternative Name	VMD2L3
Database Link	NP_689652
Function	BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]).[supplied by OMIM].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BEST3 (RC218436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BEST3.

IF Image

Anti-BEST3 mouse monoclonal antibody (TA501979) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BEST3(RC218436).

FC Image

HEK293T cells transfected with either RC218436 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BEST3 antibody(TA501979), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-BEST3 antibody(TA501979),(Red), compared to a nonspecific negative control antibody,(Blue).

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Anti-BEST3 TrueMAB Antibody clone 2H3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data