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Anti-BECN1 TRUEMAB Antibody clone 3F3
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for BECN1 (NM_003766)
|Full length human recombinant protein of human BECN1 (NP_003757) produced in HEK293T cell. |
|Human , Rat , Monkey
|WB, IHC, IF, FC
|| WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens beclin 1, autophagy related (BECN1)|
|ATG6; beclin1; VPS30|
|Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]. |
Senescence and Autophagy
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (RC201629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BECN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502527)
Anti-BECN1 mouse monoclonal antibody (TA502527) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1(RC201629).
HEK293T cells transfected with either RC201629 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BECN1 antibody(TA502527), and then analyzed by flow cytometry.