Anti-BCAR1 Antibody, clone 1A9

Also for BCAR1 (NM_014567)

Gene Name	Homo sapiens breast cancer anti-estrogen resistance 1 (BCAR1), transcript variant 6
Synonyms:	CAS; CAS1; CASS1; CRKAS; P130Cas
Immunogen	Full length human recombinant protein of human BCAR1 (NP_055382) produced in HEK293T cell.
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 1A9	Isotype	IgG1
Species Reactivity	Human , Dog , Rat , Monkey , Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Background	BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM].
Related Pathway	EGFR1 Signaling Pathway Focal Adhesion Hemostasis

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAR1 (RC209133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAR1.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BCAR1 mouse monoclonal antibody. (TA502613)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BCAR1 mouse monoclonal antibody. (TA502613)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BCAR1 mouse monoclonal antibody. (TA502613)

IF Image

Anti-BCAR1 mouse monoclonal antibody (TA502613) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1(RC209133).

FC Image

HEK293T cells transfected with either RC209133 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BCAR1 antibody(TA502613), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-BCAR1 antibody(TA502613),(Red), compared to a nonspecific negative control antibody,(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-BCAR1 antibody(TA502613),(Red), compared to a nonspecific negative control antibody,(Blue).

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Anti-BCAR1 TrueMAB Antibody clone 1A9

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