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Anti-BAG3 Antibody EPR3515
Also for BAG3 (NM_004281)
|A synthetic peptide corresponding to residues between a.a. 90 and 120 in human Bag-3 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||IP: 1:10 - 100, WB: 1:1,000 - 10,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens BCL2-associated athanogene 3 (BAG3)|
|BAG-3; BIS; CAIR-1; MFM6|
|Bcl-2-associated athanogene 3 (Bag-3) is a member of a conserved family of cyto-protective proteins that bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone acitivity in a Hip-repressible manner. It has been shown to be prominently expressed in striated muscle and colocalizes with Z-disks. Bag-3 is not required for muscle development, but appears to be critically important for maintenance of mature skeletal muscle (1-2). Bag-3 has also been shown to have anti-apoptotic activity (3). Overexpression of Bag-3 has prevented or reduced the distraction of polyubiquitinated Hsp90/Hsp70 client proteins such as Cyclin D1, Akt, glycogen synthase 3B, and p70s6 kinase, and facilitated cell survival (4).|
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Western blot - Bag3 antibody [EPR3515]; All lanes : Anti-Bag3 antibody [EPR3515] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : MCF7 cell lysate.Lane 4 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 62 kDa.Observed band size : 80 kDa .