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Anti-BAD PHOSPHO Antibody
|The antiserum was produced against synthesized phosphopeptide derived from human BAD around the phosphorylation site of serine 134 (P-K-SP-A-G).|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:5000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|BAD (Phospho-Ser134) antibody detects endogenous levels of BAD only when phosphorylated at serine 134.
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Western blot analysis of extracts from mouse liver cells, using BAD (Phospho-Ser134) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using BAD (Phospho-Ser134) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using BAD (Phospho-Ser134) antibody.The picture on the right is treated with the synthesized peptide.