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Anti-B2M GAT Antibody Polyclonal
Generated by Genomic Immunization
|DNA immunization. This antibody is specific for the entire range of the target protein.|
|Human , Mouse
|WB, ELISA, IHC
|| WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens beta-2-microglobulin (B2M)|
|beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin|
|Beta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B2M (RC207587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B2M.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-B2M polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western Blot: beta 2 Microglobulin Antibody - Western Blot was performed using affinity purified SEQer B2M antibody, aa(21-119) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer B2M antibody, aa(21-119) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
Standard curve for ELISA analysis with B2M recombinant protein (dilution range from 0.3ng/ml to 200ng/ml) using B2M Capture Antibody (Cat# TA803600/TA803617/TA802776) at 5µg/ml and HRP conjugated B2M Detection mAb (Cat# TA590171) at 0.125µg/ml.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)