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Home Antibody All anti-B2M antibodies
UltraMAB

Anti-B2M GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590171
  • Rabbit Polyclonal beta 2 Microglobulin Antibody
100ug 325 In Stock
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WB(3)
IHC(5)
ELISA(2)
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Also for B2M (NM_004048)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the entire range of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Mouse Concentration0.93mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens beta-2-microglobulin (B2M)
Alternative Namebeta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin
Database LinkNP_004039
Entrez Gene 567 Human
Entrez Gene 12010 Mouse
FunctionBeta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B2M (RC207587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B2M.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-B2M polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
WB Image
Western Blot: beta 2 Microglobulin Antibody - Western Blot was performed using affinity purified SEQer B2M antibody, aa(21-119) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer B2M antibody, aa(21-119) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
ELISA Image
Standard curve for ELISA analysis with B2M recombinant protein (dilution range from 0.3ng/ml to 200ng/ml) using B2M Capture Antibody (Cat# TA803600/TA803617/TA802776) at 5µg/ml and HRP conjugated B2M Detection mAb (Cat# TA590171) at 0.125µg/ml.
ELISA Image
ELISA: beta 2 Microglobulin Antibody
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)

 

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