OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-B2M antibodies
UltraMAB

Anti-B2M GAT Antibody Polyclonal

Generated by Genomic Immunization

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590171 Rabbit Polyclonal beta 2 Microglobulin Antibody 100ug $325 2 Weeks
Add to Shopping Cart

spacer
WB(3)
IHC(5)
ELISA(2)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the entire range of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Mouse Concentration0.93mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens beta-2-microglobulin (B2M)
Alternative Namebeta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin
Database LinkNP_004039
Entrez Gene 567 Human
Entrez Gene 12010 Mouse
FunctionBeta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Related PathwaySecreted ProteinDruggable Genome Antigen processing and presentation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B2M (RC207587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B2M.
spacer
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-B2M polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
spacer
Western Blot: beta 2 Microglobulin Antibody - Western Blot was performed using affinity purified SEQer B2M antibody, aa(21-119) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer B2M antibody, aa(21-119) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
spacer
Standard curve for ELISA analysis with B2M recombinant protein (dilution range from 0.3ng/ml to 200ng/ml) using B2M Capture Antibody (Cat# TA803600/TA803617/TA802776) at 5µg/ml and HRP conjugated B2M Detection mAb (Cat# TA590171) at 0.125µg/ml.
spacer
ELISA: beta 2 Microglobulin Antibody
spacer
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
spacer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
spacer
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
spacer
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
spacer
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title