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Home Antibody All anti-ATP6V1F antibodies

Anti-ATP6V1F TRUEMAB Antibody Clone OTI6C11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA502322 ATP6V1F mouse monoclonal antibody, clone OTI6C11 (formerly 6C11) 100ul 379
In Stock
LC418132 ATP6V1F HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502322 Carrier-free (BSA/glycerol-free) ATP6V1F mouse monoclonal antibody, clone OTI6C11 (formerly 6C11) 100ug $450 3-4 weeks
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Also for ATP6V1F (NM_004231)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ATP6V1F (NP_004222) produced in E.coli.
Clone NameClone OTI6C11 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.44 mg/ml
Guaranteed Application *WB, FC Suggested DilutionsWB 1:200~500, FLOW 1:100
Predicted MW Explanation 13.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F (ATP6V1F), transcript variant 1
Alternative NameATP6S14; VATF; Vma7
Database LinkNP_004222
Entrez Gene 9296 Human
Entrez Gene 116664 Rat
Entrez Gene 702112 Monkey
Entrez Gene 475199 Dog
FunctionThis gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq].
Related Pathway Oxidative phosphorylationMetabolic pathwaysVibrio cholerae infectionEpithelial cell signaling in Helicobacter pylori infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1F (RC210728, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1F.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP6V1F monoclonal antibody.

FC Image
HEK293T cells transfected with either RC210728 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ATP6V1F antibody(TA502322), and then analyzed by flow cytometry.


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