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Anti-ATP1A1 PHOSPHO Antibody EPR2375(2)
Also for ATP1A1 (NM_000701)
|A phospho specific peptide corresponding to residues surrounding Tyrosine 10 of human Na+/K+ATPase a was used as an immunogen. This antibody only detects Na+/K+ATPase a phosphorylated at Tyrosine 10.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC or IHC-P.
|Homo sapiens ATPase, Na+/K+ transporting, alpha 1 polypeptide (ATP1A1), transcript variant 1|
Entrez Gene 476 Human
Entrez Gene 11928 Mouse
Entrez Gene 24211 Rat
|Na+, K+-ATPase is a heterodimeric enzyme responsible for the active maintenance of sodium and potassium gradients across the plasma membrane (1). The sodium- and potassium-dependent adenosine triphosphatase (Na+,K(+)-ATPase) maintains the transmembrane Na+ gradient to which is coupled all active cellular transport systems (2). It is well accepted that inhibition of the Na,K-ATPase in the heart, through effects on the Na/Ca exchanger, raises the intracellular Ca2+ concentration and strengthens cardiac contraction. Assessing the phenotypes of mouse hearts with genetically reduced levels of Na,K-ATPase alpha 1 or alpha 2 isoforms clearly demonstrates different functional roles for these isoforms in vivo. Results definitively illustrate a specific role for the alpha 2 Na,K-ATPase isoform in Ca2+ signaling during cardiac contraction (3).|
|TransmembraneDruggable Genome Cardiac muscle contraction|
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Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [EPR2375(2)]; All lanes : Anti-alpha 1 Sodium Potassium ATPase (phospho Y10) antibody [EPR2375(2)] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HeLa cell lysate, treated with Pervanadate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 113 kDa.Observed band size : 100 kDa .