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Anti-ATG3 TRUEMAB Antibody clone 2C12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA503161
  • ATG3 mouse monoclonal antibody,clone 2C12
  • FREE positive control: HEK293T cell transient overexpression lysate (LC411559) , 20ug
100ul 325 In Stock
CF503161
  • Carrier-free (BSA/glycerol-free) ATG3 mouse monoclonal antibody,clone 2C12
100ug $450 3-4 weeks
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WB(1)
IHC(10)
IF(2)
FC(1)
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Also for ATG3 (NM_022488)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ATG3(NP_057393) produced in HEK293 cell.
Clone Nameclone 2C12 IsotypeIgG1
Species ReactivityHuman Concentration0.39 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:50~100, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens autophagy related 3 (ATG3), transcript variant 1
Alternative NameAPG3; APG3-LIKE; APG3L; PC3-96
Database LinkNP_071933
FunctionAutophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG3 (RC203453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG3.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503161)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503161)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503161)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503161)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503161)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503161)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503161)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503161)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503161)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503161)
IF Image
Anti-ATG3 mouse monoclonal antibody (TA503161) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3(RC203453).
IF Image
Immunofluorescent staining of HeLa cells using anti-ATG3 mouse monoclonal antibody (TA503161).
FC Image
HEK293T cells transfected with either RC203453 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ATG3 antibody(TA503161), and then analyzed by flow cytometry.

 

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