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Also for ARF1 (NM_001024228)
|Recombinant fragment contain a sequence corresponding to a region within amino acids 1 and 162 of ARF1 (Uniprot ID#P84077)|
|Human, Mouse (Predicted: Rat, Dog, Pig, Sheep, Rhesus Monkey, Bovine)
|WB, IHC, IF
||ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography.
|Seq homology of immunogen across species: Rat (100%), Dog (100%), Pig (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%)
|Homo sapiens ADP-ribosylation factor 1 (ARF1), transcript variant 2|
|OTTHUMP00000035715; ADP-ribosylation factor 1|
Entrez Gene 375 Human
Entrez Gene 11840 Mouse
Entrez Gene 64310 Rat
Entrez Gene 696052 Monkey
Entrez Gene 100856517 Dog
|ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]|
| Vibrio cholerae infection|
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Sample (50 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. TA308576 diluted at 1:10000.
Sample (30 ug of whole cell lysate). A: A431 . B: H1299. 12% SDS PAGE. TA308576 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft, using ARF1(TA308576) antibody at 1:100 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, using ARF1(TA308576) antibody at 1:200 dilution.