FGF4 Rabbit Polyclonal Antibody

CAT#: AP01123PU-N

FGF4 rabbit polyclonal antibody, Aff - Purified

Size: 50 ug 100 ug

Conjugation: Unconjugated Biotin


USD 399.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Indirect ELISA: To detect FGF-4 by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant FGF-4.
Sandwich ELISA: To detect FGF-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml is required. In conjunction with Biotinylated Anti-Human FGF-4 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant FGF-4.
Western Blot: To detect FGF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant FGF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (> 98 %) recombinant human FGF-4
Specificity This antibody detects FGF-4.
Formulation PBS, pH 7.2
State: Aff - Purified
State: Sterile filtered lyophilized Ig fraction
Reconstitution Method Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml.
Purification Immunoaffinity chromatography
Conjugation Unconjugated
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens fibroblast growth factor 4 (FGF4)
Background FGF4 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF4 signals through the FGFR 1c, 2c, 3c, and 4.
Synonyms Fibroblast growth factor 4, HST-1, HST, HSTF1, KS3
Reference Data
Protein Families Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway, Transmembrane
Protein Pathways MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton

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