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Anti-ANXA5 Antibody EPR3980
Also for ANXA5 (NM_001154)
|A synthetic peptide corresponding to residues in human Annexin V was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB: 1:10000 - 1:50000; FC: 1:100 - 1:1000; ICC/IF: 1:500 - 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens annexin A5 (ANXA5)|
|ANX5; ENX2; PP4; RPRGL3|
|Annexin V (ANV) is a calcium-dependent glycoprotein with a potent anticoagulant capacity in vitro, mainly as a result of its negatively charged membrane phospholipids, and capable of inhibiting the prothrombinase and Tensa complexes to reduce plaque adhesion and aggregation. Circulating ANV can be released from the cells of the vascular wall (endothelial cells, smooth muscles cells) or from secretor cells of the spleen and liver. Once it is in the plasma, it binds to blood cells (platelets and erythrocytes) or to endothelial cells (1). Annexin V is also a Ca(2+) dependent membrane-binding protein protein that forms voltage-dependent Ca2+ channels in phospholipid bilayers (2). Structural features suggest that Annexin V attaches with its convex face to membrane by specific calcium mediated interactions with at least three phospholipids. The adjacent membrane bilayer may thus become locally disordered and permeable to allow calcium inflow through the central polar channel of the molecule.|
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Western blot - Annexin V antibody [EPR3980]; All lanes : Anti-Annexin V antibody [EPR3980].Lane 1 : HeLa cell lysate.Lane 2 : Fetal kidney lysate.Lane 3 : Fetal brain lysate.Lane 4 : Jurkat cell lysate.Lane 5 : JAR cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 36 kDa.
Immunocytochemistry/ Immunofluorescence - Annexin V antibody [EPR3980]; Immunofluorescent staining of HeLa cells using 1/500 ab108194.
Immunocytochemistry/ Immunofluorescence - Annexin V antibody [EPR3980]; Immunofluorescent staining of staurosporin-treated apoptotic HeLa cells using 1/250 ab108194.
Flow Cytometry - Anti-Annexin V antibody [EPR3980]; Overlay histogram showing HeLa cells stained with ab108194 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.