EGFR Mouse Monoclonal Antibody [Clone ID: B1D8]
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Product Data | |
Clone Name | B1D8 |
Applications | FC, IP |
Recommended Dilution | Affinity Purification (For coupling, order Antibody without BSA). Blocks Ligand-induced Activation of EGFR. Inhibits Proliferation of A431 Cells (Order Antibody without Sodium Azide). Arrests Tumor Growth in vivo (Order Ab without BSA and Azide). Immunoprecipitation: 1-2 µg/500 µg protein lysate. Flow Cytometry: 0.5-1 µg/million cells. Positive Control: A431 cells, Breast or Bladder cancer |
Reactivities | Human |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Microsomes from A431 cells. |
Specificity | This antibody reacts with the extracellular domain of EGFR and blocks the EGF/TGFα−induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. Cellular Localization: Cell surface. |
Formulation | 10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide |
Concentration | lot specific |
Purification | Protein A/G Chromatography |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C. |
Stability | Shelf life: one year from despatch. |
Predicted Protein Size | ~170 kDa (wild type) and ~145 kDa (vIII variant) |
Gene Name | epidermal growth factor receptor |
Database Link | |
Background | EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas. |
Synonyms | Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1 |
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