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Home Antibody All anti-ALDOB antibodies

Anti-ALDOB TRUEMAB Antibody Clone OTI3E10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502909 ALDOB mouse monoclonal antibody, clone OTI3E10 (formerly 3E10) 100ul $325 In Stock
LC400007 ALDOB HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502909 Carrier-free (BSA/glycerol-free) ALDOB mouse monoclonal antibody, clone OTI3E10 (formerly 3E10) 100ug $450 3-4 weeks
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WB(1)
IHC(2)
FC(1)
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Also for ALDOB (NM_000035)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ALDOB(NP_000026) produced in HEK293 cell.
Clone NameClone OTI3E10 IsotypeIgG1
Species ReactivityHuman Concentration0.2 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 39.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens aldolase B, fructose-bisphosphate (ALDOB)
Alternative NameALDB; ALDO2
Database LinkNP_000026
Entrez Gene 229 Human
FunctionFructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq].
Related PathwayDruggable Genome Glycolysis / GluconeogenesisPentose phosphate pathwayFructose and mannose metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDOB (RC220062, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDOB.
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ALDOB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502909)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ALDOB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502909)
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HEK293T cells transfected with either RC220062 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ALDOB antibody(TA502909), and then analyzed by flow cytometry.
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