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Home Antibody All anti-ALDOB antibodies

Anti-ALDOB Antibody EPR3137

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SKU Description Amount Price Availability*  
TA307760
  • Rabbit monoclonal antibody against Aldolase B (clone EPR3137 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400007) , 20ug Explanation
100ul $325 3-7 Days
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WB(1)
IHC(5)
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Also for ALDOB (NM_000035)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Aldolase B was used as an immunogen.
Clone NameEPR3137 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsIHC-P: 1:100 - 1:250; WB: 1:1000 - 1:2000
Predicted MW Explanation 39 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC/IF or IP.

Reference Data

Target NameHomo sapiens aldolase B, fructose-bisphosphate (ALDOB)
Alternative NameALDB; ALDO2
Database LinkNP_000026
Entrez Gene 229 Human
Entrez Gene 230163 Mouse
Entrez Gene 24190 Rat
FunctionAldolase B is one of three known aldolase isoenzymes, and is found in kidney and small adult intestine where it is associated with aldolases A or C. Aldolase B catalyzes the reversible cleavage of fructose 1-phosphate into dihydroxyacetone phosphate and glyceraldehyde. It is involved in both glycolytic and gluconeogenic pathways, where its quaternary structure is critical for maintaining its full catalytic function. Hereditary fructose intolerance (HFI), a potentially fatal human autosomal recessive disease of carbohydrate metabolism, results with Aldolase B deficiency. Partially active Aldolase B, however, has been reported in an HFI individuals, which supports the hypothesis that adequate gluconeogenesis/glycolysis is maintained in HFI patients by the presence of partially active enzymes (1-4).
Related PathwayDruggable Genome Glycolysis / GluconeogenesisPentose phosphate pathwayFructose and mannose metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-ALDOB antibody [EPR3137]; All lanes : Anti-ALDOB antibody [EPR3137] at 1/1000 dilution.Lane 1 : Human fetal liver cell lysate.Lane 2 : Human fetal kidney cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat Anti-rabbit HRP at 1/2000 dilution.Predicted band size : 39 kDa.Observed band size : 40 kDa .
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDOB antibody [EPR3137]; Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human fetal liver tissue staining ALDOB with TA307760 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDOB antibody [EPR3137]; Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human adult liver tissue staining ALDOB with TA307760 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ALDOB antibody [EPR3137](TA307760); Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using TA307760 showing -ve staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ALDOB antibody [EPR3137](TA307760); Immunohistochemical analysis of paraffin embedded normal Human breast tissue using TA307760 showing -ve staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ALDOB antibody [EPR3137](TA307760); Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using TA307760 showing +ve staining.
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