Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein.Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormonesand plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylatedserum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbuminwhich has an N-terminal peptide that is removed before the nascent protein is released from therough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles toproduce the secreted albumin.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALB (RC205943, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALB.