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Anti-AKT2 TRUEMAB Antibody Clone OTI4G1
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for AKT2 (NM_001626)
|Full length human recombinant protein of human AKT2 (NP_001617) produced in HEK293T cell.|
||WB 1:2000, IP 2ug/500ul
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (RC217733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2.
Immunoprecipitation(IP) of AKT2 by using TrueMab monoclonal anti-AKT2 antibodies (Negative control: IP without adding anti-AKT2 antibody.). For each experiment, 500ul of DDK tagged AKT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-AKT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.