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Anti-AKT2 Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA302204 Rabbit Polyclonal Antibody against AKT2 (N-term) 100ug $325 In Stock
LC419836 AKT2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenThis AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the N-terminal region of human AKT2.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman, Mouse Concentration0.25 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000, IHC: 1:50~100, IF: 1:10~50, FC: 1:10~50
Predicted MW Explanation 55769 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 2 (AKT2), transcript variant 1
Database LinkNP_001617
Entrez Gene 208 Human
Entrez Gene 11652 Mouse
FunctionAKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of anti-AKT2 Antibody (N-term) Pab (Cat.#TA302204) in mouse liver tissue lysates (35ug/lane). AKT2(arrow) was detected using the purified Pab.
Western blot analysis of anti-AKT2 Antibody (N-term) Pab (Cat.#TA302204) in 293 cell line lysates transiently transfected with the AKT2 gene (2ug/lane). AKT2(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with AKT2 Antibody (N-term)(Cat.#TA302204), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of AKT2 Antibody (N-term)(Cat#TA302204) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
AKT2 Antibody (N-term) (Cat. #TA302204) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


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