OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-AKT1S1 antibodies

Anti-AKT1S1 PHOSPHO Antibody EPR6262-107

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310182
  • Rabbit Monoclonal antibody against PRAS40 pT246 (AKT1S1) (Phospho-specific)
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
spacer
Also for AKT1S1 (NM_032375)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Threonine 246 of human PRAS40 was used as an immunogen. This antibody only detects PRAS40 phosphorylated at Threonine 246.
Clone NameEPR6262-107 IsotypeTissue culture supernatant
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100
BufferDoes not react with Rat. Is unsuitable for ICC/IF or IHC-P.
Purification pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Note Is unsuitable for ICC/IF or IHC-P.

Reference Data

Target NameHomo sapiens AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 1
Alternative NameLobe; PRAS40
Database LinkNP_115751
FunctionPRAS40 is a novel substrate of Akt, the phosphorylation of which leads to the binding of this protein to 14-3-3 (1). It is a subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1 (2).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-PRAS40 (phospho T246) antibody [EPR6262]; All lanes : Anti-PRAS40 (phospho T246) antibody [EPR6262-107] at 1/1000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate, treated with Lambda Phosphatase.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti-rabbit at 1/2000 dilution.Predicted band size : 27 kDa.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title