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Home Antibody All anti-AKT1 antibodies
UltraMAB

Anti-AKT1 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590196
  • Rabbit Polyclonal AKT1 Antibody
100ug $325 In Stock
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WB(4)
IHC(15)
IF(1)
ELISA(1)
DB(2)
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Also for AKT1 (NM_005163)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration1mg/ml
Guaranteed Application *WB, ELISA, IHC, IF, DB Suggested Dilutions WB 1:5000~20000, IHC 1:150, IF 1:500,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1
Alternative NameAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Database LinkNP_005154
FunctionAKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Related Pathway
Apoptosis
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
Jak-STAT signaling pathway
MAPK signaling pathway
Signaling by GPCR
Toll-like receptor signaling pathway
Wnt Signaling Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (RC220257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AKT1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
WB Image
Western Blot: AKT1 Antibody - performed using affinity purified SEQer AKT1 antibody, aa(389-474) antibody. The lanes contain 5-30ug of a whole cell extract. The protein was detected as represented by the band shown.
WB Image
Western Blot: AKT1 Antibody - Cell lysate from HeLa cells were subjected to SDS-PAGE and transfer to nitrocellulose membrane. The membrane was incubated with anti-AKT antibody (1:1000 dilution) and then probed anti-rabbit IgG-HRP. Signals were acquired with a CCD camera. Arrow points to unmodified AKT1. The two bands above the arrow are probably post-translational modified AKT1. Data courtesy of a SDI customer.
ELISA Image
ELISA: AKT1 Antibody
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196)
IF Image
Immunofluorescent staining of HeLa cells using anti-AKT1 rabbit polyclonal antibody (TA590196).
DB Image
Dot Blot: AKT1 Antibody - The anti-AKT antibody (Left quadruplicate spots at each panel) or control antibody (right quadruplicate spots at each panel) were printed on membranes at 10 ng/spot. The membranes were incubated with biotinylated cell lysate (50 ug per membrane), followed by probing with streptavidin-HRP and CCD camera image acquisition. (a), Cell lysate from undifferentiated mouse embryonic stem cells; (b), Cell lysate from differentiated mouse embryonic stem cells. Data courtesy of a SDI customer.
DB Image
Dot Blot: AKT1 Antibody - Protein samples (upper left quadruplicate spots: bovine gamma globulin; upper right quadruplicate spots: cell lysate from 293 cell over expressing Nanog; below left quadruplicate spots: cell lysate from 293 cell over expressing Sox2; below right quadruplicate spots: cell lysate from 293 cell over expressing POU5F1) were printed on membranes at 10 ng/spot. The membrane was incubated with anti-AKT antibody (1:1000 dilution) and then probed anti-rabbit IgG-HRP. Signals were acquired with a CCD camera. Data courtesy of a SDI customer.

 

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