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Home Antibody All anti-AKT1 antibodies

Anti-AKT1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319278
  • Sheep polyclonal anti-AKT antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC423056) , 20ug
100ug 325 3-7 Days
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WB(1)
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Also for AKT1 (NM_001014431)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenAnti-AKT antibody was affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human AKT1 protein.  A residue of cysteine was added to facilitate coupling.
Clone Name Isotype
Species Reactivityhuman Concentration0.9 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000, IHC: user optimized, IF: user optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308.  AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways.  AKT Exhibits tight control over cell proliferation and cell viability.  Overexpression or inappropriate activation of AKT is noted in many types of cancer.   AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.  AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3
Alternative NameAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Database LinkNP_001014431
Function
Related Pathway
Apoptosis
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
Jak-STAT signaling pathway
MAPK signaling pathway
Signaling by GPCR
Toll-like receptor signaling pathway
Wnt Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western Blot of Rabbit Anti-AKT Antibody. Lane 1: HeLa cells. Lane 2: H2O2 treated MCF7 cells. Lane 3: Recombinant AKT. Lane 4: A431 cells. Load: 35 µg per lane. Primary antibody: AKT antibody at 1:300 for overnight at 4°C. Secondary antibody: IRDYE800™ conjugated Dnky-a-Sheep IgG was used at a 1:10,000 dilution for 45 min at room temperature. Block: 1% BLOTTO overnight at 4°C. Predicted/Observed size: ~55-60 kDa for AKT . Other band(s): unspecific.

 

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