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Home Antibody All anti-AK4 antibodies

Anti-AK4 TRUEMAB Antibody Clone OTI2B12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503306 AK4 mouse monoclonal antibody, clone OTI2B12 (formerly 2B12) 100ul $325 In Stock
LC402259 AK4 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503306 Carrier-free (BSA/glycerol-free) AK4 mouse monoclonal antibody, clone OTI2B12 (formerly 2B12) 100ug $450 3-4 weeks
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WB(2)
IHC(5)
IF(1)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human AK4(NP_037524) produced in HEK293 cell.
Clone NameClone OTI2B12 IsotypeIgG1
Species ReactivityHuman , Dog , Monkey Concentration0.42 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 25.1 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens adenylate kinase 4 (AK4), transcript variant 2
Alternative NameAK 4; AK3; AK3L1; AK3L2
Database LinkNP_037542
Entrez Gene 205 Human
Entrez Gene 698560 Monkey
Entrez Gene 489554 Dog
FunctionThis gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq].
Related PathwayDruggable Genome Purine metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK4 (RC220572, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK4.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK4 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503306)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503306)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503306)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503306)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503306)
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Anti-AK4 mouse monoclonal antibody (TA503306) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AK4(RC220572).
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HEK293T cells transfected with either RC220572 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK4 antibody(TA503306), and then analyzed by flow cytometry.
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