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Anti-AK4 TRUEMAB Antibody Clone OTI1H1
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Full length human recombinant protein of human AK4(NP_037542) produced in HEK293 cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens adenylate kinase 4 (AK4), transcript variant 2|
|AK 4; AK3; AK3L1; AK3L2|
Entrez Gene 205 Human
|This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq].|
|Druggable Genome Purine metabolismMetabolic pathways|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK4 (RC220572, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK4.
Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-AK4 monoclonal antibody.(1:200)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503199)
Anti-AK4 mouse monoclonal antibody (TA503199) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AK4(RC220572).
HEK293T cells transfected with either RC220572 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK4 antibody(TA503199), and then analyzed by flow cytometry.