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Home Antibody All anti-AK1 antibodies

Anti-AK1 TRUEMAB Antibody clone 4A1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500361
  • AK1 (Adenylate Kinase 1) mouse monoclonal antibody, clone 4A1
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400171) , 20ug Explanation
100ul 379 In Stock
CF500361
  • Carrier-free (BSA/glycerol-free) AK1 mouse monoclonal antibody, clone 4A1
100ug $450 3-4 weeks
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WB(2)
IF(1)
FC(1)
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Also for AK1 (NM_000476)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human AK1 (NP_000467) produced in HEK293T cell.
Clone Nameclone 4A1 IsotypeIgG1
Species ReactivityHuman, Monkey Concentration0.89 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:1000~2000, IF 1:100, FLOW 1:100
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens adenylate kinase 1 (AK1)
Alternative NameHTL-S-58j
Database LinkNP_000467
Entrez Gene 203 Human
Entrez Gene 706338 Monkey
FunctionAdenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (RC215130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK1 monoclonal antibody.
IF Image
Immunofluorescent staining of HT29 cells using anti-AK1 mouse monoclonal antibody (TA500361).
FC Image
HEK293T cells transfected with either RC215130 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK1 antibody(TA500361), and then analyzed by flow cytometry.

 

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