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UltraMAB

AFP UltraMAB Antibody, Clone UMAB66

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Related Products Product Documents
SKU Description Datasheet Price Availability*  
UM500056
  • AFP mouse monoclonal antibody, clone UMAB66, 100 ul
468 In Stock Add to Shopping Cart
UM570056
  • AFP mouse monoclonal antibody, clone UMAB66, 30 ul
$120 In Stock Add to Shopping Cart
WB(1)
IHC(1)
IF(1)
10K-CHIP(1)
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Also for AFP (NM_001134)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens alpha-fetoprotein (AFP)
SynonymsFETA; HPAFP
ImmunogenFull length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone UMAB66 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsIHC 1:100, IF 1:100
BackgroundThis gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008].

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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody (Clone UMAB66).
IHC Image
Immunohistochemical staining of paraffin-embedded Human embryonic liver tissue using anti-AFP mouse monoclonal antibody. (UM500056)
IF Image
Immunofluorescent staining of HepG2 cells using AFP mouse monoclonal antibody (UM500056, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
10K-CHIP Image
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-AFP mouse monoclonal antibody (UM500056). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

 

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