Home Antibody All anti-AFP antibodies
|A synthetic peptide corresponding to residues in C-terminus of human AFP was used as an immunogen.|
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Is unsuitable for Flow Cyt,ICC/IF or IP.
|Homo sapiens alpha-fetoprotein (AFP)|
|Alpha-fetoprotein (AFP) is a serum glycoprotein protein produced in the liver or yolk sac of fetal staged mammals. AFP synthesis is minimal after birth and trace amount is expressed in the adult liver (1). AFP gene expression is regulated by the interactions between steroid hormone receptors and transcriptional factors in separate signal transduction pathways (2). AFP functions as a binding and transporting ligand and cell growth regulator. Elevated expression level of AFP has been implicated in colorectal, ovarian, pancreatic, testicular, and certain liver cancers. High level of AFP is also seen some diseases, such as hepatitis and colitis. AFP is used as a screening marker for fetal abnormalities in pregnant women, such as Down syndrome. Recently, AFP has been introduced as an anti-cancer drug-ligand carrier, transporting drugs to target tumor cells, increasing anti-tumor efficiency (3). |
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Western blot - alpha 1 Fetoprotein antibody; Anti-alpha 1 Fetoprotein antibody at 1/50000 dilution + HepG2 cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled, at 1/2000 dilution.Predicted band size : 69 kDa.Observed band size : 69 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody; TA300675 staining alpha 1 Fetoprotein in Mouse embryonic liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 2.5% serum for 20 minutes at 23Â°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/750 in 1% BSA in TBS) for 1 hour at 23Â°C. An undiluted HRP-conjugated Horse anti-rabbit polyclonal was used as the secondary antibody.