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Anti-ADRBK1 Antibody Y137
Also for ADRBK1 (NM_001619)
|A synthetic peptide corresponding to residues in the C-terminus of human ß-ARK1 was used as an immunogen. The antibody does not cross-react with other GPRK family member.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:500; IHC-P: Use at an assay dependent dilution; ICC: 1:100 - 1:250; FC: 1:50; IP: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Homo sapiens adrenergic, beta, receptor kinase 1 (ADRBK1)|
|BARK1; BETA-ARK1; GRK2|
|The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]. |
Signaling by GPCR
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Western blot - GRK2 antibody [Y137]; Anti-GRK2 antibody [Y137] at 1/500 dilution + U937 cell lysate.Predicted band size : 80 kDa.Observed band size : 80 kDa.
Immunohistochemistry (Paraffin-embedded sections) - GRK2 antibody [Y137]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab32558 at a dilution of 1/100 - 1/250
Flow Cytometry - GRK2 antibody [Y137]; Overlay histogram showing HEK293 cells stained with ab32558 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.