OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-ADRBK1 antibodies

Anti-ADRBK1 Antibody Y137

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303385
  • Rabbit monoclonal antibody against B-ARK1 (GRK2)(Y137)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419839) , 20ug Explanation
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(1)
FC(1)
spacer
Also for ADRBK1 (NM_001619)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-terminus of human ß-ARK1 was used as an immunogen. The antibody does not cross-react with other GPRK family member.
Clone NameY137 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:500; IHC-P: Use at an assay dependent dilution; ICC: 1:100 - 1:250; FC: 1:50; IP: 1:80
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction

Reference Data

Target NameHomo sapiens adrenergic, beta, receptor kinase 1 (ADRBK1)
Alternative NameBARK1; BETA-ARK1; GRK2
Database LinkNP_001610
Entrez Gene 156 Human
Entrez Gene 110355 Mouse
Entrez Gene 25238 Rat
FunctionThe product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq].
Related Pathway
Signaling by GPCR

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - GRK2 antibody [Y137]; Anti-GRK2 antibody [Y137] at 1/500 dilution + U937 cell lysate.Predicted band size : 80 kDa.Observed band size : 80 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - GRK2 antibody [Y137]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma using TA303385 at a dilution of 1/100 - 1/250
FC Image
Flow Cytometry - GRK2 antibody [Y137]; Overlay histogram showing HEK293 cells stained with TA303385 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title