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Anti-ACVR1B Antibody EPR4815
Also for ACVR1B (NM_004302)
|A synthetic peptide corresponding to residues in the extracellular domain of human ACVR1B was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000; FC: 1:100 - 1:500; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens activin A receptor, type IB (ACVR1B), transcript variant 1|
|ACTRIB; ACVRLK4; ALK4; SKR2|
|ACVR1B is an activin A type IB receptor. Activins are dimeric growth and differentiation factors that belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases, which include at least two type I and two type II receptors. ACVR1B is a type I receptor that is essential for signaling. Mutations in this protein are associated with pituitary tumors.|
Cytokine-cytokine receptor interaction
MAPK signaling pathway
TGF Beta Signaling Pathway
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Western blot - Activin A Receptor Type IB antibody [EPR4815]; All lanes : Anti-Activin A Receptor Type IB antibody [EPR4815] at 1/1000 dilution.Lane 1 : Human brain tissue lysate.Lane 2 : Human fetal kidney.Lane 3 : SH-SY5Y cell lysate.Lane 4 : U87-MG cell lysate.Lane 5 : Human fetal liver.Lysates/proteins at 10 µg per lane.Predicted band size : 57 kDa.Observed band size : 52 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Activin A Receptor Type IB antibody [EPR4815]; ab109300, at 1/500 dilution, staining Activin A receptor type IB in Human kidney tissue by immunohistochemistry.
Immunocytochemistry - Anti-Activin A Receptor Type IB antibody [EPR4815]; Alexa Fluor 488 goat anti-rabbit IgG was used as the secondary antibody. The cells (SH-SY5Y) were fixed with 2% paraformaldehyde for 20 min.
Flow Cytometry - Anti-Activin A Receptor Type IB antibody [EPR4815]; Overlay histogram showing HEK293 cells stained with ab109300 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.