Home Antibody All anti-ACTN4 antibodies
Also for ACTN4 (NM_004924)
|Recombinant fragment corresponding to a region within amino acids 206 and 542 of alpha Actinin 4 (Uniprot ID#O43707)|
|Human, Mouse (Predicted: Rhesus Monkey, Bovine)
|WB, IHC, IF
||ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography.
|Seq homology of immunogen across species: Rhesus Monkey (100%), Bovine (100%)
|Homo sapiens actinin, alpha 4 (ACTN4)|
|ACTININ-4; FSGS; FSGS1|
Entrez Gene 81 Human
Entrez Gene 60595 Mouse
Entrez Gene 693666 Monkey
| Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]|
|Druggable Genome Focal adhesionAdherens junctionTight junctionLeukocyte transendothelial migrationRegulation of actin cytoskeletonSystemic lupus erythematosusMore Pathways >> |
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Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308476 diluted at 1:1000.
alpha Actinin 4 antibody [N2C1], Internal detects alpha Actinin 4 protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5% SDS-PAGE. alpha Actinin 4 antibody [N2C1], Internal (TA308476) dilution: 1:3000.
Sample (30 ug of whole cell lysate). A: A431 . B: Hep G2 . 7.5% SDS PAGE. TA308476 diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. TA308476 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(TA308476) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4(TA308476) antibody at 1:200 dilution.