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Home Antibody All anti-ACTN2 antibodies

Anti-ACTN2 Antibody EP2529Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303379
  • Rabbit monoclonal antibody against Alpha-Actinin 2 (EP2529Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC420189) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
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Also for ACTN2 (NM_001103)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus of human Alpha-Actinin 2 was used as an immunogen.
Clone NameEP2529Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:40; IHC-P: 1:100 - 1:250; FC: 1:100
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for ICC/IF.

Reference Data

Target NameHomo sapiens actinin, alpha 2 (ACTN2), transcript variant 1
Alternative NameCMD1AA
Database LinkNP_001094
FunctionAlpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq].
Related Pathway
Adherens junction
Focal Adhesion
Hemostasis

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Sarcomeric Alpha Actinin antibody [EP2529Y]; All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] at 1/20000 dilution.Lane 1 : Human skeletal muscle lysate.Lane 2 : Rat heart lysate.Lane 3 : Human heart lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 103 kDa.Observed band size : 103 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sarcomeric Alpha Actinin antibody [EP2529Y]; TA303379 at 1/100 dilution staining Sarcomeric Alpha Actinin in human muscles by Immunohistochemistry, Paraffin-embedded tissue.

 

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