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Also for ACTC1 (NM_005159)
|Recombinant fragment contain a sequence corresponding to a region within amino acids 99 and 354 of alpha Actin (cardiac muscle) (Uniprot ID#P68032)|
|Human, Mouse (Predicted: Chicken, Pig, Xenopus laevis, Bovine, Xenopus Tropicalis)
|WB, IHC, IF
||ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:10000
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography.
|Seq homology of immunogen across species: Xenopus laevis (100%), Xenopus Tropicalis (100%), Pig (100%), Chicken (100%), Bovine (100%)
|Homo sapiens actin, alpha, cardiac muscle 1 (ACTC1)|
|ACTC; ASD5; CMD1R; CMH11; LVNC4|
Entrez Gene 70 Human
Entrez Gene 11464 Mouse
|Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq]|
| Cardiac muscle contractionHypertrophic cardiomyopathy (HCM)Dilated cardiomyopathy|
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alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein by western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 10 % SDS-PAGE. alpha Actin (cardiac muscle) antibody (TA308841)
Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. TA308841 diluted at 1:1000.
Sample(30 ug of whole cell lysate). A:HeLa S3. B:MOLT4. 12% SDS PAGE. TA308841 diluted at 1:500
Immunohistochemical analysis of paraffin-embedded Gastric CA, using alpha Actin (cardiac muscle)(TA308841) antibody at 1:100 dilution.
alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min. Green: alpha Actin (cardiac m