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Home Antibody All anti-ACTC1 antibodies

Anti-ACTC1 Antibody EP184E

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303378
  • Rabbit monoclonal antibody against Actin (Pan)(EP184E)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417479) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(3)
IF(1)
FC(1)
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Also for ACTC1 (NM_005159)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Actin was used as an immunogen. This Actin antibody recognizes all muscle actin.
Clone NameEP184E IsotypeIgG
Species ReactivityMouse, Rat, Cow, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1,000; IHC: 1:100 200; ICC: 1:250 500; FC: 1:20; IP: 1:20
Predicted MW Explanation 42 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens actin, alpha, cardiac muscle 1 (ACTC1)
Alternative NameACTC; ASD5; CMD1R; CMH11; LVNC4
Database LinkNP_005150
Entrez Gene 70 Human
Entrez Gene 11464 Mouse
Entrez Gene 29275 Rat
FunctionActins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq].
Related Pathway Cardiac muscle contractionHypertrophic cardiomyopathy (HCM)Dilated cardiomyopathy

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - muscle Actin antibody [EP184E]; Anti-muscle Actin antibody [EP184E] at 1/1000 dilution + A431 cell lysate at 10ug/lane.Secondary.Goat anti-rabbit, HRP labelled. at 1/2000 dilution.Predicted band size : 42 kDa.Observed band size : 42 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - muscle Actin antibody [EP184E]; Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin TA303378 at a 1/100 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E]; Fluorescent immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using TA303378. Green-specific fluorescent staining.
IHC Image
Immunohistochemistry (Frozen sections) - Anti-muscle Actin antibody [EP184E]; Immunohistochemical analysis of rat kidney tubule tissue, staining muscle Actin with TA303378.
IF Image
Immunocytochemistry/ Immunofluorescence - muscle Actin antibody [EP184E]; Immunofluorescent staining of HeLa cells using TA303378 at a 1/250 dilution.
FC Image
Flow Cytometry-Anti-muscle Actin antibody [EP184E](TA303378); Overlay histogram showing HeLa cells stained with TA303378 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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